View Colony Pcr Gel PNG. Molecular cloning techniques require some method of screening colonies for the presence or furthermore, the convenient ready mix format, including the option of a red dye for direct gel loading. Pcc 6803 samples, the pcr of the template and the application on a agarosegel are described.

Yeast colony pcr is used to amplify fragments from the yeast genome as well as transformed plasmids. Pcc 6803 samples, the pcr of the template and the application on a agarosegel are described. • use the following protocol for colony screening by pcr if the cloned pcr fragment is shorter than 3 kb.

Run 5ml on agarose gel to check for amplification.

For multiple samples, make a large master mix and aliquot 50 µl in each pcr tube (also on ice). Streamline your colony pcr protocol to mere minutes. Load 12µl of each sample. Colony pcr is the modification of the conventional pcr in which the bacterial colonies are directly used as a after the completion of the colony pcr reaction, the pcr products are run on the 2% agarose gel.