View Colony Formation Assay Crystal Violet PNG. I am having a bit of trouble counting colonies on my soft agar assay. Colonies are fixed with glutaraldehyde (6.0% v/v), stained with crystal violet (0.5% w/v) and counted using a stereomicroscope.

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Materials for colony formation in hard agar assay. Aureus isolates obtained from a veterinary teaching hospital during. Wash excess crystal violet with dh2o and allow dishes to dry.

In this method colonies are stained with crystal violet or methylene blue after seedind in 6 well at the end, stain the cells with crystal violet.

Stain the colonies with crystal violet and count them if so desired. Can anyone please send me protocol for colony formation assay ( not soft agar gel colony assay). Incubate assay at 37°c in optional: Staining colonies with crystal violet.