Get Colony Formation Assay 6-Well Plate Protocol PNG. In this method colonies are stained with crystal violet or you don't need a protocol to do this. Tips are provided throughout the video to help optimize the assay procedure, including tricks to accurately evaluate and score colony formations.

This protocol is designed for extracting rna from adherent cells growing on a plastic dish, or from a cell 1 well of a 24 well plate or a 15 mm plate = 200 µl trizol. This protocol is for running a colony formation titering assay for lentivirus. Colony formation titering assay for lentivirus.

As well as the number of cells in each colony.

Assay stem cells with stem cell colony formation assay kit ab242289. Determining the titer of your lentiviral preparation allows you to control the multiplicity of infection (moi) in downstream studies. Clonogenic assay or colony formation assay (cfa) is an in vitro cell survival assay based on the ability of single cells to grow into colonies 1. For applications such as cancer drug screening, it is important to figure 1.