Download Colony Pcr Protocol Background. Or could try this instead (from biotechniques. Jump to navigationjump to search.

Protocols
Protocols from biomod19.biocat.tu-berlin.de
Prepare your pcr reactions in 0.2 ml pcr tubes (single or strip tubes): Jump to navigationjump to search. We have recently learned that this protocol is not reliable for any pcr product larger than ~300 bp.

Colony pcr e 4 part a:

Setting up colony pcr reactions is nearly identical to preparing a standard pcr reaction: I simply put some fungal cells (once again, the. Primers are used which generate a pcr product of known size. 5 microliters 10x colony pcr buffer.